Analysis Note

Positive ControlHeLa cells

Application

Immunoblotting (2.5-5 µg/ml)r>Immunocytochemistry (2.5-5 µg/ml)r>Paraffin Sections (not recommended)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.

Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Immunogen

a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jensen, D.E., et al. 1991. Oncogene6, 1219.Reiss, M., et al. 1990. Cancer Res.50, 6641.RouscherIII, F.J., et al. 1988. Science240, 1010.Sassone-Corsi, P., et al. 1988. Cell54, 553.Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.Verma, I.M., et al. 1987. Cancer Res.49, 29.Verma, I.M., et al. 1987. Cell51, 513.Muller, R. 1986. Viochem. Biophys. Acta.823, 207.Verma, P. 1986. Trends Genet.2, 93.Greenberg, M., et al. 1984. Nature (London)311, 433.

Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

Packaging

200 µg in Glass bottle

Physical form

Lyophilized from a volatile buffer, 200 µg BSA.

Reconstitution

Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.

Warning

Toxicity: Standard Handling (A)